Khushali Upadhyay

10 Questions 14 Answers 0 Followers

Questions related from Khushali Upadhyay

Which method is good for extract preparation?

I am working on one plant for that I require to prepare methanolic and aqueous extract of leaf. Which method I have to use for that? I have to do cell line testing as well as the characterization...

10 January 2020 1,443 3 View

What is the Short term effect of Antiestrogenic Molecular treatment on TGF beta level in case of MCF-7 Cells ?

If we treat MCF--7 cells with Tamoxifen and 4-OH Tamoxifen drug for 5days. and then we are going to check a TGF-Beta level in this cells and compare with non treated cells. Dose it make any...

02 April 2015 6,873 1 View

On the MCF-7 cell growth, what is the effect of complete growth medium (especially EMEM) with phenol red and with out phenol red ?

We have got less cell confluency (60%) in case of With out phenol red EMEM . WE can not use with phenol red EMEM media as we are performing the effect of Tamoxifen drug on Estrogenicity of cell...

18 March 2015 7,994 5 View

Can somebody elaborate the cross talk between receptors of nuclear hormone receptor and growth hormone receptor super families?

I want to study the cross talk between these two super family in case breast cancer. Thank you.

13 March 2015 6,987 3 View

How can we check the expression of phosphorylated molecules in vitro?

During any drug treatment, if any change in signaling is going on, the expression of phosphorylated molecules will be checked, according to my knowledge. But how is it possible?

21 February 2015 4,815 5 View

Does anyone know how to pick a colony of MCF-7 from the agar plate after doing Soft Agar Colony Assay?

We are performing Single Cell Colony Formation Assay of MCF-7 cells in our Dissertation Project work.We found the colony after 20 days of experiment. We want to separate out the colony from the...

19 February 2015 9,320 4 View

Why should we do sample activation in In house ELISA method For MCF-7 cell line for evaluating TGF beta 1?

Why we should do Sample activation in In house ELISA method For MCF-7 cell line for evaluating TGF beta 1 ??

10 February 2015 3,620 3 View

How can I compare ELISA Techniques for growth factor TGF beta 1 estimation?

We will going to perform Estimation of Elevated level of cellular TGF beta 1 in MCF-7 cell line after giving the treatment of Tamoxifen (10^-6M). For that we are choosing ELISA technique.(Indirect...

04 February 2015 8,565 4 View

Do you know any destaining method for better colony observation of MCF-7 cells?

We are performing any soft agar colony assay. We use crystal violate stain. But now we would like to destain our plate and then observe the single cell colony of MCF-7 cell line. Can you please...

04 February 2015 6,370 3 View

Are there any problems if we use media EMEM without Phenol red for the MCF-7 cell line and In Vitro Cell culture?

We are going to perform growth curve , cytotoxicity assay,protein estimation,ELISA, Anti cancer drug (Tamoxifen) effect on cell population and Soft agar colony Assay. We are using without...

02 February 2015 8,565 10 View