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Questions related from Khushali Upadhyay
I am working on one plant for that I require to prepare methanolic and aqueous extract of leaf. Which method I have to use for that? I have to do cell line testing as well as the characterization...
10 January 2020 1,437 3 View
If we treat MCF--7 cells with Tamoxifen and 4-OH Tamoxifen drug for 5days. and then we are going to check a TGF-Beta level in this cells and compare with non treated cells. Dose it make any...
02 April 2015 6,866 1 View
We have got less cell confluency (60%) in case of With out phenol red EMEM . WE can not use with phenol red EMEM media as we are performing the effect of Tamoxifen drug on Estrogenicity of cell...
18 March 2015 7,988 5 View
Why we should do Sample activation in In house ELISA method For MCF-7 cell line for evaluating TGF beta 1 ??
10 February 2015 3,613 3 View
We will going to perform Estimation of Elevated level of cellular TGF beta 1 in MCF-7 cell line after giving the treatment of Tamoxifen (10^-6M). For that we are choosing ELISA technique.(Indirect...
04 February 2015 8,560 4 View
We are performing any soft agar colony assay. We use crystal violate stain. But now we would like to destain our plate and then observe the single cell colony of MCF-7 cell line. Can you please...
04 February 2015 6,365 3 View
We are going to perform growth curve , cytotoxicity assay,protein estimation,ELISA, Anti cancer drug (Tamoxifen) effect on cell population and Soft agar colony Assay. We are using without...
02 February 2015 8,555 10 View
