Khushali Upadhyay

9 Questions 13 Answers 0 Followers

Questions related from Khushali Upadhyay

Which method is good for extract preparation?

I am working on one plant for that I require to prepare methanolic and aqueous extract of leaf. Which method I have to use for that? I have to do cell line testing as well as the characterization...

10 January 2020 1,439 3 View

What is the Short term effect of Antiestrogenic Molecular treatment on TGF beta level in case of MCF-7 Cells ?

If we treat MCF--7 cells with Tamoxifen and 4-OH Tamoxifen drug for 5days. and then we are going to check a TGF-Beta level in this cells and compare with non treated cells. Dose it make any...

02 April 2015 6,868 1 View

On the MCF-7 cell growth, what is the effect of complete growth medium (especially EMEM) with phenol red and with out phenol red ?

We have got less cell confluency (60%) in case of With out phenol red EMEM . WE can not use with phenol red EMEM media as we are performing the effect of Tamoxifen drug on Estrogenicity of cell...

18 March 2015 7,990 5 View

Can somebody elaborate the cross talk between receptors of nuclear hormone receptor and growth hormone receptor super families?

I want to study the cross talk between these two super family in case breast cancer. Thank you.

13 March 2015 6,983 3 View

Does anyone know how to pick a colony of MCF-7 from the agar plate after doing Soft Agar Colony Assay?

We are performing Single Cell Colony Formation Assay of MCF-7 cells in our Dissertation Project work.We found the colony after 20 days of experiment. We want to separate out the colony from the...

19 February 2015 9,316 4 View

Why should we do sample activation in In house ELISA method For MCF-7 cell line for evaluating TGF beta 1?

Why we should do Sample activation in In house ELISA method For MCF-7 cell line for evaluating TGF beta 1 ??

10 February 2015 3,615 3 View

How can I compare ELISA Techniques for growth factor TGF beta 1 estimation?

We will going to perform Estimation of Elevated level of cellular TGF beta 1 in MCF-7 cell line after giving the treatment of Tamoxifen (10^-6M). For that we are choosing ELISA technique.(Indirect...

04 February 2015 8,561 4 View

Do you know any destaining method for better colony observation of MCF-7 cells?

We are performing any soft agar colony assay. We use crystal violate stain. But now we would like to destain our plate and then observe the single cell colony of MCF-7 cell line. Can you please...

04 February 2015 6,366 3 View

Are there any problems if we use media EMEM without Phenol red for the MCF-7 cell line and In Vitro Cell culture?

We are going to perform growth curve , cytotoxicity assay,protein estimation,ELISA, Anti cancer drug (Tamoxifen) effect on cell population and Soft agar colony Assay. We are using without...

02 February 2015 8,559 10 View