Why RP-HPLC method is important as a lot release method (LRA) for monoclonal antibodies in addition to SEC, WCX, CE_SDS and SDS-PAGE?
Help me to draw graph of Bioconcentration
10 June 2024 4,941 2 View
I want to estimate total sugars in carrot and products developed from it. I have been using lane and eynon method. I could get the values for reducing sugars but not for total sugars. What is the...
28 May 2024 9,382 4 View
My name is kiranbir kaur and m student of MSC Computer Engineering in Solent University Southhampton (United Kingdom).Please suggest me the topic for dissertation.Include the Problem and Solution.
24 May 2024 4,355 2 View
Hi, My name is kiranbir kaur and m student of MSC Computer Engineering in Solent University Southhampton (United Kingdom).Please suggest me the topic for dissertation.Include the Problem and Solution.
24 May 2024 1,180 3 View
Dear researchers, I am treating the waste water by vermifiltration process using different filter bed media. After the treatment, BOD, COD, TSS were found to be reduced but reduction in total...
18 May 2024 1,897 3 View
what is the main reason of formation of bonding in between two atoms
16 May 2024 3,607 0 View
Using 10 ml of 1% methanolic hcl, 1 g fresh sample and OD at 530 and 657nm
07 May 2024 4,440 1 View
I am planning to study the overexpressed pattern of lncRNA. Could you explain whether the primer designing procedure for cloning a long non-coding RNA (lncRNA) gene follows the same approach as...
14 April 2024 3,966 3 View
when i am installing cooja 3.0 in ubuntu 18.04 i am facing some problem give me some solution
01 April 2024 4,305 0 View
I have searched for the methods where I do not require any cell or cell line, but I could not find it. I want to check the anticancer property of the given compound (protein) without using cells....
21 March 2024 10,021 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...
27 July 2024 8,508 0 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...
18 July 2024 10,010 0 View