I am working on Molecular identification (gene COI) of parasitoids (Chalcid Wasps). After DNA isolation by using Nucleospin kit, Gel check confirmed bands for most of the samples. But, PCR bands are missing as confirmed by gel check. Universal primers have been used.
If you ran gel with your DNA extraction samples, the bands you've seen are genomic DNA. PCR samples should show bands for the target gene only, so apparently the PCR didn't work. Even though these are universal primers, they might work at different PCR settings for different species. You may try to change annealing temperature/DNA or primer concentration.. Hope that might help!
I agree with Alina. I presume by now you've already checked papers for primers and PCR protocols successfully used for the part of Chalcidoidea in question. Perhaps there are primers designed to target this taxon more specifically. I would go for those, if such can be found.
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