How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

Can I use video interview excerpts from others in public domain (YouTube, Vimeo, etc) for my empirical analysis?

Dear community, I am currently writing my master's dissertation. As a part of the method, I have to include views (interview) of experts for the empirical analysis. The problem is I have not...

31 January 2021 3,438 3 View

Having the moderator continuous or dichotomous? Using MANOVA or the Process macro (Hayes)?

Hi community! Currently finalising my Master thesis research. I manipulated my independent variable to have three conditions. Originally, I proposed a 3(3 conditions of independent v)x2...

30 January 2021 7,821 6 View

Could one suggest a common formula for Penicillium Chrysogenum spore counting by spectroscopy? and also its nm?

I am working with this strain but I do not have the formula for spore counting. please just suggest a spectrophotometric method.

05 January 2021 4,308 3 View

How the year 2021 can be made good for global community?

Many steps can be taken to make the year 2021 good for humanity. For instance, if powerful and rich countries decide and spend some part of their resources for the improvement and betterment of...

30 December 2020 830 4 View

What was the most worst thing and what was the most beneficial thing that happened for global community in 2020?

Many bad and good things happened for global community in 2020. However, the present question is about one top most worst thing and one top most good thing that happened in the world in 2020 and...

30 December 2020 6,262 3 View

What are health and agricultural econmic impact of the herbicide 2,4-dichlorophenoxyacetic_acid (2,4-D) in your country?

Dear Research community, I am writing a review about 2,4-D. Google Scholar has identified 11000 publications with this herbicide as a keyword since 01/01/2018. There is plenty for me to read....

29 December 2020 1,145 6 View

Any papers related to the topic of extraction of optical properties of tissue-mimicking phantoms?

Dear Community, I'm trying to find some information about the extraction of optical properties of phantom without the usage of spectrophotometer. Thanks in advance

21 December 2020 6,770 3 View

How to decolonize climate change research?

Who has the right to define what is climate change research should be? Who should define the climate change research topic, research questions, and research design? Who should own the climate...

29 November 2020 1,077 12 View