Hi, What could be the possible reasons for my Sf-9 insect cell culture not showing proper growth, despite using fresh Corning fetal bovine serum (FBS) that was heat-inactivated for 30 minutes at 56°C before being aliquoted? What factors should I consider to troubleshoot this issue, such as media preparation, cell handling, or other potential stressors that might be affecting the cell growth?

Thanks,

Muhammad