Muhammad Hafeez

16 Questions 6 Answers 0 Followers

Questions related from Muhammad Hafeez

Should L-glutamine be supplemented in Grace’s Insect Medium for Sf9 cell culture?

I am currently culturing Spodoptera frugiperda (Sf9) cells using Grace’s Insect Medium and wanted to clarify whether it is necessary to supplement the medium with L-glutamine for optimal cell...

16 June 2025 1,086 1 View

Guidance on Designing eGFP Primers for tagging and Cloning Strategy for Insect Receptor Localization?

Hello Everyone, I am planning to design primers for eGFP to fuse with our target insect receptor gene. The goal is to clone this construct into an expression plasmid and transfect it into an...

17 December 2024 4,554 2 View

Unhappy Sf9 cells and resembling crystalline and tadpole-like shapes ?

I am currently working with Sf9 cells and have been testing various growth conditions. Specifically, I’ve tried using Insectagro SF9 medium supplemented with different concentrations of FBS (3%,...

04 November 2024 3,024 0 View

Why My Sf-9 cells are not growing properly?

Hi, What could be the possible reasons for my Sf-9 insect cell culture not showing proper growth, despite using fresh Corning fetal bovine serum (FBS) that was heat-inactivated for 30 minutes at...

18 October 2024 6,959 1 View

Efficiency of my primers, why there are measurable values appearing in the negative control wells, where no cDNA was added?

Hello, I hope you're doing well. I’m currently analyzing the results from my qRT-PCR experiment, particularly focusing on the efficiency of my primers. However, I’ve noticed something concerning:...

13 September 2024 9,685 3 View

Why Cell lines showing a high/low luminescent signal in the cAMP assay?

Hi I am Muhammad and working with an Sf9 cell line expressing neuropeptide receptors and using the cAMP-Glo™ Assay kit to measure the luminescent signal of these receptors. One of my cell lines is...

30 April 2024 8,709 0 View

How to make a lysis buffer for the extraction DNA from insects?

hello @ all researchers, i want to extract DNA from the midgut of insect so i need to know how to make a lysis buffer..? thanks

09 March 2018 1,865 3 View

protocol for lysis buffer and TE buffer for the extraction of DNA from insects .?

I need the protocol for lysis buffer and TE buffer for the extraction of DNA from insects Thanks

09 March 2018 4,017 3 View

How to make a p-nitrophenol standard curve which use to obtain the molar extiction coefficients..?

how to make a p-nitrophenol standard curve which use to obtain the molar extiction coefficients to measure the P450 PNOD activities..

03 August 2017 9,357 0 View

Effects of Gossypol on Fitness Costs Associated with Resistance to Bt Cotton in Pink Bollworm

Hello, every one i need this paper.

29 June 2017 2,367 2 View

hello to every one, I need the large number of Spodoptera exigua larvae for bioassy expriment. I am searching for a suitable diet.?

I need the large number of Spodoptera exigua larvae for bioassy expriment. I am searching for a suitable diet.?

27 May 2017 4,531 2 View

Toxicity of spinosad and lufenuron on different biological parametes of spotted bollworm or other lepidopterous insects.?

I need research articles which related to lethal and sub lethal effect f spinosad and lufenuron on different biological parameters of lepidopterous insects

12 April 2016 1,620 2 View

Why my cell lines is showing a high/low luminescent signal in the cAMP assay

01 January 1970 7,010 0 View

Unhappy Sf9 cells?

I am currently culturing Sf9 insect cells for a protein expression and noticed some tadpole-like cells (red circled in the picture). They seem to reduce/disappear when cells are close to...

01 January 1970 5,010 1 View

In my fusion cloning construct, I noticed that instead of the expected stop codon 'TAA' at the end of the EGFP tag?

Hi, @ALL In my fusion cloning construct, I noticed that instead of the expected stop codon 'TAA' at the end of the EGFP tag, the sequence includes an extra nucleotide"C", resulting in 'TCAA'. What...

01 January 1970 1,496 1 View

After digesting the piB/V5-TOPO plasmid (4200 bp) with StuI, which has a single StuI recognition site, how many bands should appear on the gel for the

Hi, After digesting the piB/V5-TOPO plasmid (4200 bp) with StuI, which has a single StuI recognition site, how many bands should appear on the gel for the linearized plasmid? Thanks

01 January 1970 422 2 View