16 Questions 6 Answers 0 Followers
Questions related from Muhammad Hafeez
I am currently culturing Spodoptera frugiperda (Sf9) cells using Grace’s Insect Medium and wanted to clarify whether it is necessary to supplement the medium with L-glutamine for optimal cell...
16 June 2025 1,080 1 View
Hello Everyone, I am planning to design primers for eGFP to fuse with our target insect receptor gene. The goal is to clone this construct into an expression plasmid and transfect it into an...
17 December 2024 4,553 2 View
I am currently working with Sf9 cells and have been testing various growth conditions. Specifically, I’ve tried using Insectagro SF9 medium supplemented with different concentrations of FBS (3%,...
04 November 2024 3,014 0 View
Hi, What could be the possible reasons for my Sf-9 insect cell culture not showing proper growth, despite using fresh Corning fetal bovine serum (FBS) that was heat-inactivated for 30 minutes at...
18 October 2024 6,951 1 View
Hello, I hope you're doing well. I’m currently analyzing the results from my qRT-PCR experiment, particularly focusing on the efficiency of my primers. However, I’ve noticed something concerning:...
13 September 2024 9,681 3 View
Hi I am Muhammad and working with an Sf9 cell line expressing neuropeptide receptors and using the cAMP-Glo™ Assay kit to measure the luminescent signal of these receptors. One of my cell lines is...
30 April 2024 8,691 0 View
hello @ all researchers, i want to extract DNA from the midgut of insect so i need to know how to make a lysis buffer..? thanks
09 March 2018 1,862 3 View
I need the protocol for lysis buffer and TE buffer for the extraction of DNA from insects Thanks
09 March 2018 4,011 3 View
how to make a p-nitrophenol standard curve which use to obtain the molar extiction coefficients to measure the P450 PNOD activities..
03 August 2017 9,351 0 View
Hello, every one i need this paper.
29 June 2017 2,360 2 View
I need the large number of Spodoptera exigua larvae for bioassy expriment. I am searching for a suitable diet.?
27 May 2017 4,529 2 View
I need research articles which related to lethal and sub lethal effect f spinosad and lufenuron on different biological parameters of lepidopterous insects
12 April 2016 1,614 2 View
01 January 1970 7,004 0 View
I am currently culturing Sf9 insect cells for a protein expression and noticed some tadpole-like cells (red circled in the picture). They seem to reduce/disappear when cells are close to...
01 January 1970 5,005 1 View
Hi, @ALL In my fusion cloning construct, I noticed that instead of the expected stop codon 'TAA' at the end of the EGFP tag, the sequence includes an extra nucleotide"C", resulting in 'TCAA'. What...
01 January 1970 1,491 1 View
Hi, After digesting the piB/V5-TOPO plasmid (4200 bp) with StuI, which has a single StuI recognition site, how many bands should appear on the gel for the linearized plasmid? Thanks
01 January 1970 418 2 View