Hello everyone,

I have some issues with my wester-blot PVDF membrane.

First I transfered my proteins to the membrane with the Trans-blot turbo from Qiagen and hybridized my antibodies. Everything worked fine and I obtained clear bands. I then stripped my membrane and hybridized another antibody which worked too. But after drying my membrane and reactivating it, it turns yellow/green (a little bit fluorescent) and no band appears anymore, even after a new hybridization. This happened 3 times so I wonder if anyone can explain this ? Did the exposure to the sun be a suitable hypothesis?