What would be the cause of a smeared bacterial gDNA band? Could not changing tips between loading a negative control after Loading DNA sample lead to a faint band of same size ( just trying to find the cause of the faint band since no reagent was contaminated)? what can I do to improve my gel.
1 % gel 95 V for 60 minutes 1kb ladder
Maccus Hao Jie Wong
you have to change tip for each loading. especially loading control. not changing tips could have contaminated your stock/working control.
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Yuan-Yeu Yau
Sherine Ahmed
1.
Load negative tube first, then the sample tubes. I leave one empty lane between the negative lane and positive lane.
2. As Laurence Stuart Dawkins-Hall
mentioned, that is not really a 'smear', and should have no concern.
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