Sherine Ahmed

3 Questions 1 Answers 0 Followers

Questions related from Sherine Ahmed

Question about adding positive and negative controls in sequencing?

I am about to use sequence a set of genes in human DNA samples using ion torrent and Illumina to find variants. Do I need a positive control ? to make sure that the sequences were sequenced...

26 January 2022 9,743 3 View

How to determine the volume of insert to add for a ligation reaction?

For the ligation reaction, the mass of insert can be calculated : (mass vector x size of insert in kilobase / size of vector in kilobase) x (insert:vector). How to then convert the ng into volume...

09 May 2021 5,068 2 View

Why my PCR amplified DNA sequence appear to be smeared on agarose gel?

What would be the cause of a smeared bacterial gDNA band? Could not changing tips between loading a negative control after Loading DNA sample lead to a faint band of same size ( just trying to...

13 April 2021 3,669 2 View