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Questions related from Maccus Hao Jie Wong
Hi all, I have been trying to look to understand which promoters are recognized by e.coli and which are not. I have difficult in finding the list of promoters. Currently I am using 5xUAS-E1B...
10 May 2021 3,074 1 View
when I did plasmid purification from TOPO blunt cloned with mCherry insert only, I found that the DNA yield is relatively lower than other clones by 2/3 (its highly reproducible results I don't...
21 April 2021 9,302 2 View
I have similar colony numbers for my self-ligation (EcoRV + Cla1) and my re-ligation (EcoRv+BamH1+Cla1 with two inserts). My No-ligation plate has no colonies at all. I wonder whats wrong with...
12 April 2021 7,817 4 View
I am doing stats analysis on the quantification of DNA damage under two conditions (light and dark) across 4 time point, the quantity was reduced across time (which seems normal) and some may have...
22 September 2018 9,594 2 View
I have run samples using two 96wells on two different run. On the second plate i re-run two samples (6 replicates) as my inter-run control. Now i have got the results and use qBASE to do inter-run...
25 April 2018 9,214 2 View
My best substitution model was estimated to be GTR+G+I using JmodelTest. in Beauti setting, all given values (including gamma shape,proportion of invariant and rate ac,ag etc) was set and...
26 January 2018 2,796 3 View
i would like to ask if anyone experiences issues with using ice-synthego lately? i have a KO mutant F1 generation and it should carry heterogenous of the KOgene. But lately the sequence i...
01 January 1970 8,864 0 View
