Hi everyone, I have recently thawed HL60 cells that had traveled in dry ice overseas and did not melt during the travel. When I first opened them they seemed to be okay and proliferating. After a while when I split them as they will be 500,000cells/ml, they started dying and I have no idea why. I have worked with this cell line before and have not experienced this situation before.
I spin down the cells, remove the medium containing dead cells but spinning does not help to remove them all, also, I am assuming that dead cells are affecting living cells stimulating their death. As the days are passing the dead cell to live cell ratio increases and I may lose them all.
What can I do at this point to save my cells from dying? Has anyone experienced a situation like this before? Thank you very much for your input.
Dear Fatma Basak Turan sorry to see that your very interesting technical question has not yet received any answers from experts in the field. As an inorganic chemist I'm certainly not a proven expert in this area, but I noticed that a closely related question has been asked earlier on RG. Perhaps some of the answers are useful for you:
Why do my HL-60 cells die in culture?
https://www.researchgate.net/post/Why_do_my_HL-60_cells_die_in_culture
(26 answers)
Another related RG question received only one answer:
How long do HL60 neutrophil-like cells survive after differentiation?
https://www.researchgate.net/post/How_long_do_HL60_neutrophil-like_cells_survive_after_differentiation
Good luck with your work and best wishes!
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