I did solid state fermentation for 7 days. I used A. niger, Penicillium sp. and T. harzianum. 1 flask without fungus as control. It seems like my control (unfermented) shows higher DPPH activity. Is it possible for the fungus to use up the antioxidant compound throughout the fermentation? Anyone can suggest any paper?
Hello Carolynna, the use of the DPPH assay provides an easy and rapid way to evaluate antioxidants by spectrophotometry, so it can be useful to assess various products at a time. If the DPPH assay showed low activity, i don't see a problem. It is possible the amount of antioxidants was either low or the fermentation process reduced it. I don't know if you ran the assay before, during or after fermentation. Look at these gaps and we could talk later. Cheers!
Maurice Ekpenyong i did the assay after fermentation. And i used non fermented as my control.
Jai Ghosh, i used sago residual as the substrates and i don’t have any specific target compound.
Is it possible for my extracts to be degraded? Dissolved my extracts in Methanol.
Hello Carolynna
Nice question.the fungi in question has feruloyl esterase activity ....so it should be able to liberate some antioxidant precursors such as hydroxycinnamic acids......and reduce possibly cellulose structures and
Other structural polysaccharides via their respective enzymes it's innately possess.....
Kindly note this; there are free phenolic compounds which can easily be removed probably per your protocol and also bound phenolic compound which must also be extracted which I believe methanol alone would not help. Irrespective of the compound of interest remember this...soluble or free phenolic compounds and insoluble/bound phenolic compounds.....meanwhile take note of total phenolic compounds release during Fermentation as Sir Maurice Ekpenyong and it would be nice if you select any compound of interest from the hydroxycinnamic acids.....
All the best
Is this fermentation is in aerobic and non-aerobic conditions pH of the medium and medium compositions ( is there any radical scavenging substrates) , dilution ? so there are many things one has to get clear conditions
Hellie Gonu thank you for your comment. Do you have any paper to suggest.
- Badges
- Science topic
- Similar topics
- Human-Computer Interaction
- Collaboration