Can we perform immnuhohistochemistry (IHC) with frozen tissue?

Dear colleagues I am planning to use anti-caspase 3 active in frozen brains. Have you performed that? Because I only find when tissues are paraffin-embedded. i will appreciate your answer and...

16 January 2020 5,712 2 View

Which sample, from an alive mouse, is better for western blot?

I need to perform a western blot from mice samples, to select positive animals for my experiments. I was thinking of doing western blot from ear tissue, finger or tail. Which one is better? Thanks

31 December 2018 6,432 4 View

What should I do to get my attB PCR product?

Hello, I am workig with three elements Multisite Gateway Kit to clone a promoter, gene and terminator. Now I have good results for the promoters and terminator, even I could add attB regions for...

20 May 2018 7,299 4 View

Still is important to measure metals in particulate matter less than 10 microns in scientific research?

31 December 2016 8,363 2 View

Can someone help me run an Energy Decomposition Analysis (EDA)?

I'm trying to identify the types of interactions that take place between a Surface Group in an Activated Carbon and a pollutant, to determine what fraction of this interaction energy is...

10 November 2014 6,705 9 View

What is the difference between pointwise similarity and structural similarity in trajectories?

With respect a match from object.

22 October 2013 9,317 1 View

Problems with flow of Particle sizer model SMPSS 3936 NL88-N - can you help?

Our research group just bought a SMPSS 3936 NL88-N. We are having some problems because this equipment came without their neutralizer. Does anyone bought this equipment without this accessory? Our...

09 October 2013 8,521 7 View

Problems with flow of Particle sizer model SMPSS 3936 NL88-N - can you help?

Our research group just bought a SMPSS 3936 NL88-N. We are having some problems because this equipment came without their neutralizer. Does anyone bought this equipment without this accessory? Our...

30 September 2013 5,839 6 View

Has this interpretation of the Mach's principle already been explored ?

Excerpt from https://en.m.wikipedia.org/wiki/Mach%27s_principle :

01 January 1970 6,855 7 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

No title

25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View