Hello Everyone,

Has anyone faced issues with the degradation of Matrigel after 6 days of spheroid culturing, especially when changing the medium after 3-4 days? Regarding this matter, I have repeated the experiment more than 20 times, carefully checking the pH, temperature, and medium composition each time. Interestingly, I achieved proper spheroid formation only once, but subsequent experiments under the same conditions consistently resulted in degradation.

I kindly request everyone to provide suggestions or share experiences if you have encountered similar situations. Your insights would be greatly appreciated.

Thank you