Anuj Singh

15 Questions 33 Answers 0 Followers

Questions related from Anuj Singh

How to make anaerobic suspension from Anaerobic bacterail capsules?

I am planing an experiment where I need to dose Anaerobic bacteria into mouse. For this I need to make anaerobic suspension from capsulated anaerobic bateria. I do not have anaerobic lab. How to...

23 December 2023 1,307 4 View

Anaerobic Bacteria Viability?

I have collected mouse fecal matter and stored at minus 80 degree C. I want to culture the gut microbiome in fecal matter and then will do MALDI-TOF MS for speciese and strain identification. Can...

04 December 2023 9,789 6 View

Mouse Short Chain Fatty Acid Quantification by ELISA methods?

I have mouse serum samples. In this I want to quantify short chain fatty acids like acetate propoinate and butyrate.. Please suggest the cheapest methods to quantify it (like ELISA?)

18 June 2023 3,470 5 View

Anaerobic Bacteria Viability?

I am working on a probiotic that have anaerobic bacteria spp. This probiotic is available in capsules commercially from Seeking Health LLC USA. I need to dissolve the capsule powder in anaerobic...

07 January 2023 2,956 2 View

How to isolate slow growing clone from a cancer cell line with LDC ; Traditional method without involving cell shorter?

Hi, I want to isolate slow growing clones from a cancer cell line using LDC method. Anyone can help me in this, it would be a great help for me. I do not have cell sorter and the cell line is...

13 February 2022 8,659 0 View

How the tumor necrosis affect efficacy in syngenic tumor model?

We all know that almost all syngenic tumor are very prone to be necrosed once the the tumor volume reaches 1000 mm^3. Anyone have any idea how to run the efficacy beyond 1000 mm^3 tumor without...

09 August 2021 3,301 2 View

Is Durvralumab induce PD-L1 Receptor Internalization?

I read many article about Durvalumab Ab. I got it blocks PD-L 1 and inhibit its binding to PD-1. Anyone have any idea that it also induces PD-L receptors inteenalization along with blocking the...

06 March 2021 1,002 0 View

KLN 205 Syngenic tumor model ?

Hi Everyone, I need a help from you people . Actually I am trying to develop KLN205 renal cancer syngenic tumor model . I have used both Balb/c mice and C57BL/6 mice and 1 and 2 million of KLN205...

06 February 2017 9,448 6 View

How much possibility is there that mycoplasma will infect murine tumor cell line ?

If anyone have any adea about the corelatiob between mycoplasma and murine tumor cell line like B16F10, CT26 and 4T1 Anuj

13 August 2016 4,629 6 View

How many 4T1 cells are needed to develop a subcutaneous tumor in Balb C mice?

I want to develop 4T1 tumor model. How many cells are needed to develop a subcutaneous tumor in Balb C mice? Should I can use male Balb C mice in place of female? If anyone have any clue about...

27 November 2015 6,608 5 View

How can I make single cell suspension from xenograft mouse tumors?

I want to make a single cell suspension from xenograft tumor and inject the same in another set of mice to make a reproducible tumor model. Could anyone share the protocol with me for the same! I...

01 July 2015 2,479 5 View

Will reinjection of a tumor cell in the same set of mice produce a tumor or not ?

Previously I have injected U87 in female Nude mice 5 million /mouse with matrigel. Tumor came in all injected animals but only few tumor started to grow and most of the other tumor started to...

01 July 2015 6,487 5 View

Could anyone tell me that if I inject evans blue dye (20mg/kg-5mL/kg) into lung of mice, will the mice survive or not for at least one week?

Thanks in advance for your replies.

07 May 2015 4,772 4 View

How do I develop a orthotopic GBM model using the U87 cell line?

Please can any one help me to get the coordinates ( x, y and z from bregma ) to inject the cells for orthotopic GBM Model development using U87 cell line?

23 December 2014 898 1 View

End point parameters in Orthotopic Lung Tumor Model

Hi Folks Hope all is well with you. I need a help from you. I have LLC orthotopic tumor model. We have implanted cell in left lung as usual. We have given treatment. End point parameters are :...

01 January 1970 6,941 0 View