13 Questions 28 Answers 0 Followers
Questions related from Anuj Singh
I am planing an experiment where I need to dose Anaerobic bacteria into mouse. For this I need to make anaerobic suspension from capsulated anaerobic bateria. I do not have anaerobic lab. How to...
23 December 2023 1,239 4 View
I have collected mouse fecal matter and stored at minus 80 degree C. I want to culture the gut microbiome in fecal matter and then will do MALDI-TOF MS for speciese and strain identification. Can...
04 December 2023 9,736 6 View
I have mouse serum samples. In this I want to quantify short chain fatty acids like acetate propoinate and butyrate.. Please suggest the cheapest methods to quantify it (like ELISA?)
18 June 2023 3,413 5 View
I am working on a probiotic that have anaerobic bacteria spp. This probiotic is available in capsules commercially from Seeking Health LLC USA. I need to dissolve the capsule powder in anaerobic...
07 January 2023 2,898 2 View
We all know that almost all syngenic tumor are very prone to be necrosed once the the tumor volume reaches 1000 mm^3. Anyone have any idea how to run the efficacy beyond 1000 mm^3 tumor without...
09 August 2021 3,264 2 View
I read many article about Durvalumab Ab. I got it blocks PD-L 1 and inhibit its binding to PD-1. Anyone have any idea that it also induces PD-L receptors inteenalization along with blocking the...
06 March 2021 964 0 View
Hi Everyone, I need a help from you people . Actually I am trying to develop KLN205 renal cancer syngenic tumor model . I have used both Balb/c mice and C57BL/6 mice and 1 and 2 million of KLN205...
06 February 2017 9,391 6 View
If anyone have any adea about the corelatiob between mycoplasma and murine tumor cell line like B16F10, CT26 and 4T1 Anuj
13 August 2016 4,594 6 View
I want to develop 4T1 tumor model. How many cells are needed to develop a subcutaneous tumor in Balb C mice? Should I can use male Balb C mice in place of female? If anyone have any clue about...
27 November 2015 6,563 5 View
I want to make a single cell suspension from xenograft tumor and inject the same in another set of mice to make a reproducible tumor model. Could anyone share the protocol with me for the same! I...
01 July 2015 2,445 5 View
Thanks in advance for your replies.
07 May 2015 4,739 4 View
Please can any one help me to get the coordinates ( x, y and z from bregma ) to inject the cells for orthotopic GBM Model development using U87 cell line?
23 December 2014 868 1 View
Hi Folks Hope all is well with you. I need a help from you. I have LLC orthotopic tumor model. We have implanted cell in left lung as usual. We have given treatment. End point parameters are :...
01 January 1970 6,905 0 View