Protein size measurement using Zetasizer?

The idea is to measure the hydro- protein sizes in various solvents (eg. with high ionic strength, EtOH/Water, other organic/water solvents), then, due to the extremely low solubility of some...

28 January 2021 297 3 View

How many times should I inject the drug before I take samples for RNA-Sequencing?

I'm going to perform RNA-sequencing of the zebrafish brain with three different treatments, which are naive fish, alcohol withdrawal fish injected with Saline, alcohol withdrawal fish injected...

08 December 2020 4,643 2 View

How do I transform the "Transcripts" into the "Gene symbol"?

I downloaded the expression matrix data "GSE143416_Brain_data_TPM.txt.gz" of GSE143416 from Gene expression omnibus ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi). However, the first column of...

30 November 2020 1,304 3 View

Why can't I get the same natural frequencies, which are calculated by the exported [K] and [M] matrices, compared to the ANSYS Modal analysis result?

First of all, I created a simple solid cubic structure with coarse mesh in ANSYS WB Modal modulus. With the boundary condition is set as 1 face fixed, the first 5 natural frequencies (fn) given by...

23 June 2020 9,836 13 View

How to use Python to optimize molecular structure in batch?

I have a batch of molecular files(.xyz) now and want to optimize geometry of these molecules in batch. Is there some advice about how to process them in batch with Avogadro by using Python? Thank...

23 February 2020 632 4 View

Has anyone observed thermal-induced twin boundary migration?

As we know, due to the tendency to decrease the overall Gibbs free energy, grain boundaries in crystalline materials are subjected to thermal-induced migration, either through a diffusive flux of...

23 December 2019 3,272 2 View

What kind of cells are suitable for cytotoxicity test of nanoparticles?

Hi everyone, I'm currently working on bone-targeting polymeric nanoparticles for treatment of osteoporosis. Just wondering what kind of human osteoblast (or other bone cells) are best suitable for...

03 October 2019 9,077 2 View

Meta-Analysis: How do you compare longitudinal studies?

I'm doing a meta-analysis in R with surgical patients. How do you do a meta-anlayis with longitudinal type studies?? We are looking at radiographic values preop, postop, and follow up (3...

26 July 2019 3,756 2 View

Can you compare AMPA/NMDA ratio between mouse strains?

I've read that you can only compare AMPA/NMDA ratio before and after drug treatment as the value itself doesn't mean anything. Is this true? Can you compare AMPA/NMDA ratio between mouse strains...

15 April 2018 3,543 1 View

Has anyone done colon cancer cell 3D culture before?

I am recently doing PDX model but find serious fibroblast cells contamination. Can anyone share experience on that one?

27 October 2017 4,438 2 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View