Do you think that Open Access journals have been made only to collect money from the authors?

Unfortunately, when the open access way was started, the objective was ethical: Open Science! but now with the emergence of hundreds of new open access journals (especially those from predatory...

10 November 2017 7,242 3 View

Do you know a simple and easy method to unify the style of references in an article citing papers manually without using a managing software?

11 December 2016 6,747 8 View

Do you know an open source software that i can use to make like these pictorials ?

08 September 2016 2,931 8 View

Can you identify these lichens (botanical names)?

I need their scientific names please, or some research teams that works on their pharmacological activities !

08 September 2016 6,684 5 View

According to your experience, what is the best model to study the antiangiogenic activity of natural products ?

03 April 2016 314 2 View

Darwin, medicine and cancer ! What do you think about this wonderful paper?

01 February 2016 348 2 View

Important for MD and PhD students !?

Dear friends, find in attched file the new list of indexed journals by Scopus database, be carefull some of "Indian journals" were indexed but now Scopus is purifying the list. Source Title...

01 February 2016 7,102 0 View

Do brain have a freshness time ?

31 December 2015 9,528 1 View

List of a predator journals ?

Here is my list , what about yours ? http://scholarlyoa.com/2014/01/02/list-of-predatory-publishers-2014/#comment-392196

11 December 2015 2,407 14 View

In 2015, students can know more information and details than their supervisors , why there is always this hierarchy that kills science and creativity?

11 December 2015 8,445 13 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View