Why is there no DNA in a sample when I crashed the mycelium cells by liquid nitrogen and stored it until extraction?

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absorption= 1-mag(S(FP1:1,FP1:1)S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)S(FP2:1,FP1:1)) is it correct for absorption ?

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MD MOE RMSD

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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fatal error

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fatal error

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Selvaraj Jagannathan

Dear Khalil during the storage of mycellium have you added cryoptotectent (DMSO) with on the liquid nitrogen, because of that .

Mohammad Ibrahim

Thanks, but do think that some cell components can degrade the DNA or there is some effect of water on DNA if stored before doing the extraction?

Ayat Ibrahiem Esmaeel Al-Laaeiby

You can keep your mycelium on deep freeze (-80)C and crash in the same day when you start extraction of DNA

Which is suitable for use with Python? MySQL or SQL Server?

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Why am I getting 'Infinity' and 'NaN' in my mixed-mode energy release values?

Do I need to ask permission to use pictures for my questionnaire?

Sample modeling in ABAQUS software and its relationship with the real world?

absorption= 1-mag(S(FP1:1,FP1:1)*S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)*S(FP2:1,FP1:1)) is it correct for absorption ?

Is there association of SARS-CoV-2 infection rate with meteorological factors?

An article on the Iranian state's civil liability in the COVID-19 matter needs supervision; Is anyone interested in collaborating?

Inter-rater / inter-coder reliability for thematic analysis?

How to define two different phases with different species?

MD MOE RMSD

MD MOE RMSD

fatal error

fatal error

fatal error

fatal error

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

How to increase a model accuracy ?

How to repeat an operation on an atom selection for multiple times in PyMol?

absorption= 1-mag(S(FP1:1,FP1:1)S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)S(FP2:1,FP1:1)) is it correct for absorption ?