On performing Zeta potential analysis of a protein in a buffer system (50mM Tris, 250mM NaCl, 6M Urea, 15mM BME, 1mM EDTA, 1mM PMSF). A canary yellow colouration was observed what could have led to that colouration?
P.S: the electrode is still functional.
John Francis Miller
Electrolysis of the electrode.
See:
https://enlightenscientific.com/2023/12/28/electrode-influence-on-zeta-potential-in-high-salt-media/
and
https://enlightenscientific.com/2023/12/27/protein-zeta-potential-analysis-impact-of-salt-and-ph/
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Ulf Nobbmann
Hi Mohammed Tabrez Banegar
you most likely produced some colloidal gold. You could try to measure without the urea and see if it still happens with native protein, the effect may be less or even disappear.
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