Why is the predicted molecular weight of the protein not authenticated by the SDS-PAGE result?

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Mangal Singh

it depends on what percentage of gel you have used- 36 and 41 seems close to me.

Determine the size with gel filtration chromatography or more easily accurately with MALDI TOF

Adesola Julius Tola

Hi !

SDS-PAGE is just only for estimation of molecular weight of proteins and not the exact value.It gives you a range at which your protein belong. You can use 12%SDS-PAGE to estimate your MW of protein.

All the best.