In many papers I see that is so popular the use of RFLP for phages studies? why not use others techniques (PCR,).
I would be interested in knowing the thermal decomposition by thermogravimetric analysis of Labrafac lipophile WL1349 since this item is not reported in the SDS by the supplier. Thanks in advance.
10 November 2023 8,388 0 View
My question emerges as I have just came across an interesting correlation (non-causal) between Blue Brain's project on 11-D neuronal structures using algebraic topology, and 10-D Calabi-Yau...
08 October 2023 2,718 1 View
Only when hemolytic anemia results in a two- to threefold or larger increase in hemoglobin catabolism does jaundice owing to rise of serum unconjugated bilirubin become noticeable. The level of...
07 September 2023 3,103 0 View
Nowadays, it has been a trend to see and hear all sorts of news about how quantum physics is revolutioning the world around us. Crystal-clear achievements and discoveries on quantum mechanics,...
15 August 2023 8,689 9 View
Hello, I am posting this question because I have not found this issue described in any EMSA problems/limitations list. My main objective is to detect the retention (electrophoretic mobility shift)...
03 July 2023 5,852 0 View
I´m haveing trouble with some samples I´m trying to determine Serpina 5 content by ELISA assay. I´m using a comercial strip kit. With my director we are starting to think the protein is forming...
07 March 2023 10,046 5 View
Crystal violet has been used since the O'Toole and Kolter model to quantify biofilm formation and I was wondering why safranin is not used.
04 January 2023 7,793 0 View
I need to get some equilibrium constants for speciation diagrams. I was planning to use HypSpec 2014 but I haven't received a reply from the e-mail given in the HyperQuad webpage. Does anyone know...
05 May 2022 5,411 3 View
I'm trying to mesure total ROS production in bone marrow derived dendritic cells with H2DCF-DA (Invitrogen), but I having some problems. After differenciated, I plate the cells to 1x10^6/ml and...
26 December 2021 3,354 3 View
I recently received the results of a fragment analysis run on a 3730xl capillary electrophoresis analyzer. I needed to use ROX 500 as the ladder in order to be able to compare this new data with...
22 December 2021 1,364 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View