Crystal violet has been used since the O'Toole and Kolter model to quantify biofilm formation and I was wondering why safranin is not used.
I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...
28 January 2021 3,867 1 View
I have trouble with exciting wavelengths higher than 460 nm while e.g. ex with 405 nm works. Filtercubes are correc, as I used them with my old xenon lamp before... I work on axiovert200M and with...
25 January 2021 5,201 2 View
I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...
20 January 2021 1,542 4 View
We'd like to use this kit for brain tissue samples (rat). When do we stop the reaction, 30, 60, and 120 minutes? We don't like to test at all three stop points, because we've too many samples.
17 January 2021 6,744 2 View
I'm having troubles in the differentiation and quantitation of each bacteria population during co-cultivation. The morphologic characteristics are very similar and it's really difficult to count...
13 January 2021 1,550 1 View
Hello, As you must know, it is now sometimes possible to have several co-first authors in the co-authorship of a scientific publication, when two persons led the paper and had equal contribution...
21 September 2020 2,995 5 View
Hello, we would like to perform oral gavage in mice. The vehicle recommended for our drug consist on 0.5% methylcellulose and 5% Dimethylacetamide (0.1 ml/ 10g mouse). Could somebody help us to...
20 August 2020 8,720 2 View
Dear all, I am planning to undertake quantitative research with a survey for my thesis. I am wondering if the hypothesis formulation is compulsory or am I able to do my research without it? My...
29 July 2020 6,145 5 View
I am very interested in the possible impact of radiation, espacially electromagnetic waves on insects. I searched several times in different online libraries, if there exists any research about...
29 April 2020 5,918 2 View
There are now many new satellites and are becoming very more ( 30 000) in the next future. I read in the internet that this satellites have not ready investigates risks on healthy and nature due...
23 April 2020 3,660 5 View
It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...
02 March 2021 728 2 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...
23 February 2021 6,723 2 View
I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.
22 February 2021 1,410 1 View
Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...
21 February 2021 5,899 4 View
I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...
14 February 2021 5,633 1 View
I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...
09 February 2021 5,155 5 View
I am using methyl violet 10B and reactive orange 16 (RO16) dyes for my Ph.D. research. I'm trying to find the molecular size for both of these dyes. It is possible for me to find these dyes'...
01 February 2021 3,333 4 View
I am looking to incorporate a unnatural amino acids which is not available in the swisssidechain database into a protein crystal structure of pdb? I am using chimera 1.14 vis. tool?
26 January 2021 4,064 3 View
Hi everyone, I hope you are all fine, As a part of my Ph.D. I'm trying to simulate eletroplastic effects on forming of titanium alloys. I'm trying to use crystal plasticity but I'm not sure if it...
20 January 2021 2,832 1 View