Why is rbcL not getting amplified in macroalgae?

More Satej Bhushan's questions See All

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Vaccination is in its First phase in India. The front line is to be vaccinated yet. In this situation opening of institutes may be dangerous for the life of children.

01 February 2021 660 2 View

How to use textures in SAR-based crop classification?

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14 December 2020 1,861 2 View

Which are the best methods to graft the polymeric nano/micro particles with lipids?

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07 December 2020 5,085 4 View

I am looking for an open source seismic hazard and risk analysis software. Is there any software available free of cost?

Open source and easy to learn with proper documentation.

26 April 2020 4,994 2 View

Can we use halogen lights as a light source in Photovoltaic module testing?

in order to calculate the electrical efficiency of a given PV module (photovoltaic module) can we use halogen lights as a source of energy for electricity generation? if yes, then what care we...

05 December 2019 7,194 5 View

Some other person with similar name claim all of my research publication on research gate . how can I reclaim my Owen publication on this site?

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15 November 2019 2,309 4 View

How to structure interview schedule for research topic on which respodent will not gave true answer or hide the truth?

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09 November 2019 8,408 2 View

How the oxidation of graphite takes place in side the nuclear reactor core?

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20 September 2018 8,273 3 View

How to know whether dopant ions are occupying interstitial sites in the lattice OR, replacing the host cations from their lattice positions?

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Is it possible to hack the bio-aging process by natural gene stimulator?

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10 January 2018 6,605 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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03 March 2021 3,568 1 View

How to increase a model accuracy ?

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03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

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02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

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How to address this issue in the analysis of Real-Time PCR results?

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01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

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28 February 2021 6,477 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Sunil Kumar Sahu

Which primer you are using for rbcl? If you can provide more details regarding PCR program and concentration of DNA then some suggestions can be made Satej.

Satej Bhushan

94C 5 min, 94C 1 min, annealing at 50C for 1.5 min, & then 72C for 2 min (35 cycles) and final extension for 7 min

concentration varies between 50-100 ng/ul

Beivy Kolondam

Hi Satej, how did you extract the DNA? How long did you do cell lysis? Were you using proteinase K? Sometimes you have lots of nuclear DNA but no chloroplast DNA. You must incubate longer time in cell lysis solution and proteinase K (approx. 1-3 hours in 55'C).