Satej Bhushan

5 Questions 11 Answers 0 Followers

Questions related from Satej Bhushan

Can anyone suggest the method/ protocol to stain colonies in Soft Agar Colony Formation Assay for Cancer cells?

I tried with Crystal violet but was not able to stain the colonies. It seemed that the stain could not penetrate the semisolid agar.

25 September 2017 6,352 7 View

Anyone have experience processing seaweed in a lab?

Seaweed grows in colonies. The habitats are also inhabited by different marine organisms both flora and fauna. While sampling these "impurities" are also included so it becomes very difficult to...

31 January 2014 3,332 9 View

Any specific method to get PCR amplifiable DNA from Padina sps?

I have tried to isolate DNA from Padina but it is not getting amplified using markers that are working fine for other algal samples. Is there any specific method or precaution which should be...

29 January 2014 9,711 3 View

Why is rbcL not getting amplified in macroalgae?

Markers like COX and ITS have worked fine for marine algae but rbcL is not getting amplified for same DNA sample. Any suggestions.

04 December 2013 8,236 3 View

Which technique can identify gene location?

How to identify whether a particular bacterial gene is chromosomal or on plasmid, before sequencing?

20 November 2013 6,339 5 View