is it stored right at hte back of a fridge where the cooling coils are. these will be very cold even if the average temperature in the fridge is higher particularly if the door seals are leaking so warm air is coming in and the fridge is cooling all the time. Possible also the thermostatic control of the fridge s not working properly
Hello Paul, it was in the door (definitely never below 4c). I am doubting if there is any relation with the concentration of TRIS.
that is very odd. Are there any other bottles of liquid in the fridge or on the door and are these freezing or is it only the tris? Is the whole bottle freezing or are just a few crystals forming. What weight of tris did you use to make the buffer or was this bought commercially please?
I never see it freeze in that temperature, what type of bottle you used or it cap? sometimes it not freezing but crystal of HCl that reacted with the cap (metal).
100 mM Tris does not freeze at 4 C. That means that either (a) you've made a major (eg factor of 10) error in the composition and it is crystallizing or (b) your refrigerator is incorrectly adjusted. Both thermostats and thermometers are routinely wrong by 2 - 3 C. I'd check the termperature with a thermometer, having first calibrated the thermometer in a mixture of ice and distilled water, which has a temperature of 0 C by definition.
thank you all, I think the problem with the thermostat indeed. Anyways Thank you.
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