After extracting DNA from soil samples using the QIAGEN DNeasy PowerMax Soil Kit the yield for these particular samples have been very low so when I tried concentrating the DNA for two different sites the DNA yield was higher than before concentration with one site but not the other on the qubit, most were lower than before concentration yet the yield was high using the nanodrop. Has anyone ran into similar issues or have tips on how to navigate this problem?
Dear Andrea,
I am not sure I understood your question correctly, but generally speaking:
1) qubit, if properly calibrated, is more precise instrument, so I would trust it more
2) if you are asking why concentrating worked for one site, and not for another - this will depend on what exactly you did to concentrate your samples
Dear Andrea,
As Albert has said, additionally
Nanodrop usually detects total nucleic acids even the degraded ones hence high readings and Qubit on the other hand detects intact nucleic acids.
I used similar extraction kit for soil samples in detecting microbes in soil. To enhance TNA concentrations, I modified the incubation period and reduced the total elution volume to 30ul depending on downstream process.
I hope this helps out.
Nanodrop measures both RNA and DNA (both absorb at 260), and also the degraded ones. Also proteins absorb at 260, but more at 280. Qubit measures only double-stranded DNA if this is the kit system that you have, you need another kit to measure RNA. Soil is a very complex material...
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