Why is LAMP performed with Bst polymerase and not other isothermal polymerases, e.g. phi29?
In order to amplify the desired DNA targets isothermally, LAMP is performed with Bst polymerase. Isothermal amplification like loop-mediated isothermal amplification (LAMP) is a DNA amplification technique that can be performed at a single temperature. LAMP is currently considered one of the most powerful isothermal amplification techniques, relying on a strand-displacement polymerase combined with four to six primers. These primers recognize several specific regions in the target DNA and two of the primers form loop structures to facilitate subsequent rounds of amplification producing high levels of DNA. Since the LAMP reaction is so robust, an extremely large amount of DNA is generated, specifically, 1 to 10 copies of DNA can be amplified to 109 to 1010 copies often within 30 minutes, producing assays with excellent sensitivity and specificity.
I know what LAMP is, my question is about the choice of isothermal polymerase. What makes Bst better than Bsm for example?
Hi Andreas,
Did you find an answer to your question from last year? I am wondering the same thing...
Bst or Bsm is the main DNA polymerase that carries out LAMP as the reaction temperature is above 60 ℃ to ensure the "DNA breathe" of the template, while phi 29 could not work under this temperature. Besides, I don't think that Bst is better than Bsm. In my experiments, Bsm showed less spurious amplification than Bst.
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