Swagat Mohanty

12 Questions 12 Answers 0 Followers

Questions related from Swagat Mohanty

Can anybody suggest me a proper protocol of antityrosinase activity for essential oils?

I am carrying out the tyrosinase activity of various essential oils taking L-Dopa as a substrate. I need to know the required concentration of the substrates and the other reagents used in this...

17 March 2022 3,937 1 View

Are essential oil components included in plant extracts?

Steam distillation produces essential oils, which are made up of volatile, typically fragrant chemicals. Extracts are made by immersing plant matter in a solvent and are commonly stated to include...

04 June 2021 6,002 5 View

How could I scale up a column for preparative isolation/purification from analytical conditions in a HPLC ?

How to determine the optimum separation conditions by using an analytical column.

22 May 2021 5,529 6 View

Which factors should be considered that mostly affect the microwave assisted extraction (MAE) ?

Currently, I am working on the antioxidant property and phenolic content of various species of Curcuma using the MAE method taking methanol as a solvent. What factors should I focus on for...

02 May 2021 1,834 4 View

How to calculate the IC50 value for DPPH and ABTS assay?

Currently, I am working on the antioxidant property of a biological sample. How to determine its IC50 value using dpph and abts assay?

10 March 2021 6,723 4 View

How much dilution would a silver nanoparticle solution need for UV-Vis and DLS characterization ?

Preparation of silver nanoparticles is being done using the citrate reduction method. It is to be characterized using UV-Vis and DLS, What should be the dilution factor for to be analyzed in a...

23 February 2021 854 0 View

What is the reason for arising of a negative peak during a sample run in an HPLC system?

What could be the reason behind a negative peak mostly arising during the initial stage of sample run (RT 1-10).

16 February 2021 4,626 4 View

How do I scale up a column for preparative isolation/purification from analytical conditions in HPLC?

What protocol should I follow to determine optimum separation conditions using an analytical column in an HPLC system?

04 February 2021 7,623 3 View

How to calculate the polysaccharide content of a curcuma species through HPLC method?

what all standards are needed to quantify and analyze the composition with a proper method fie for the mobile phase to run the sample ?

01 February 2021 8,035 4 View

How to perform a Gelatin liquefaction test for bacterial cultures ?

What would be the perfect protocol to perform this test signifying the positive and negative results?

27 January 2021 6,077 3 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

23 January 2021 3,815 3 View

How can I check the chemical composition of an essential oil using HPLC analysis?

Injected an oil having a concentration of 832mg/ml and found a flat top peak and was not able to quantify. How to resolve this problem?

21 January 2021 8,779 3 View