How much the nanoparticles reach the inflammatory tissue?

Dear colleagues, I want to know the average analysis data of EPR effect on inflammatory tissue (acute lung inflammation/injury, ischemia/reperfusion, rheumatoid arthritis, and sepsis..) In...

22 December 2020 3,147 2 View

How to measure the bonding strength of transfered graphene on PDMS substrate?

Chemical vapor deposition (CVD) was used to grow graphene on copper (Cu ) substrate, then the single layer graphene was transfered onto a PDMS substrate. I want to measure the bonding strength of...

17 October 2020 3,567 3 View

How to define the particle agglomeration in composite materials or foundry alloys and measure its size distribution quantitatively?

28 June 2020 7,614 3 View

What form of Mg will be selected during the purification of prokaryotic expression protein?

Hello everyone Recently I wanted to purify several proteins by prokaryotic expression, I read some related literature and found that Mg is needed in the protein purification buffer. I am a little...

02 May 2020 931 3 View

Free acid and its salt, can HPLC-PDA-MS tell its difference?

I have a free acid R-COOH and its salt R-COOM. Both are insoluble in water but soluble in methanol. When their methanol solutions are injected into a HPLC-PDA-MS system, are they going to have the...

06 March 2020 4,952 4 View

Sodium chloride solution saturated vapor pressure at sub zero temperature?

Can anyone suggest where to find the data or any equations related to the salt solution vapor pressure at sub-zero temperature? Many thanks!

24 December 2019 7,741 2 View

[Systematic Reviews] What to do if publication bias exist?

When doing the systematic reviews, if you I confirmed a publication bias during meta-analysis, my questions is what to do next? I though to add discussion about the publication bias, but I want...

17 November 2019 5,634 4 View

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26 October 2019 7,914 3 View

How does acridine orange works to recognize apoptosis, autophagy or lysosomal stability?

I used acridine orange staining to detect autophagy. First, the cells were grown in RPMI 10%FBS in confocal dishes, treated with compound with or without with wortmannin (0.5uM). Wortmannin was...

08 August 2019 9,319 5 View

If two shRNA linked together, it works or not?

03 August 2019 299 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View