Within the diluent of the Sigma-Aldrich assays for ABTS, they recomend BSA fraction V. What is the function of it into the process?
Thank you.
Hi there, I have transfected these two plasmids into 293T packaging cells to encapsulate a pLVX-M-Puro coding plasmid. I had already validated the stability of the pLVX-M-Puro insert by transient...
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Hi there, I am planning an experiment to isolate the ER protein fraction from a cell culture. I have found a few commercially available kits, such as...
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I was looking at a sediment sample from a "biosphere" that I´m creating and I came accross this little guy. I´m almost certain that it´s a freshwater diatom but would like to know the specific...
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Send me an e-mail for more details at [email protected]
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I've been trying to isolate both mitochondria and nuclei from the rat brains that I work with. But when I run blotting some proteins appears in every fraction of the sample, B-actin and COX4 for...
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Hi there, I want to ask whether a ratio of 260/230 of 1,55 is considered low for primer after its resuspension. Thanks in advance
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I am having problems staining this stage. In rapid search, I cannot find any paper or protocol to perform this analysis. Thanks in advance.
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Hi there, I have read that some (but not all) lentiviral transfer plasmids can be used in transient transfections to achieve transgene expression and those that can are primarily third-generation...
27 February 2023 6,184 2 View
I´ve read many papers on the matter that talk about its potential, the science behind it, etc. but haven´t found an actual production plant anywhere that actually extracts white hydrogen (derived...
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I want access to a database where proteins can be searched and the presence of ubiquitination and other parameters (ub link, mono/poly/branched ubi...)
20 December 2022 2,315 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
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Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
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Palmitic acid presence in aqueous fraction
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
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I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
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I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...
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We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Let n∈ℕ, where the function f:A⊆ℝ𝑛→ℝ, 𝐴 is Borel, and (A,d) is a completely seperable space. What is the name of the functions where the graph of f in any (n+1)-dimensional box of A×ℝ is...
17 July 2024 258 1 View