What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Has annyone try to reuse EveryBlot Blocking Buffer (Bio-rad) for fluorescence western blotting?

Hello to every one, Our lab has recently switched our western blot technique from chemiluminescence to fluorescence. Therefore, we have to optimize the blocking buffer. We have successfully try...

05 February 2021 7,886 1 View

How can I be sure that my bone marrow cells are differentiated into bone marrow derived macrophages (day 7) at a gene expression level?

Hi everyone, I'm trying to establish a standard protocol to cultivate BMDMs. I'm culturing BM cells for 7 days in 100mm petri dishes, addition of M-CSF(20ng/mL) on day 0 and day 3. I would like...

09 July 2020 1,742 3 View

What research can you do in the Physician Assistant Lab?

Physician Assistant is a health professional that work closed with the physician, the lab is essentially about performing medical procedures in teaching models. The questions what type of research...

02 March 2020 1,301 2 View

Viable cells of Saccharomyces stains very rapidly with propidium iodide. Why is that?

I am trying to see cell death in Saccharomyces cereviciae through flow citometry and propidium iodide staining. Unfortunately viable cells also stains very rapidly with propidium iodide...

06 October 2019 5,048 3 View

How to create a "Surface Kinetic File" for CHEMKIN PRO starting from a DFT derived mechanism?

How to create a "Surface Kinetic File" for CHEMKIN PRO starting from a mechanism derived by Density Functional Theory? The DFT mechanism has several elementary reactions involving molecular...

11 June 2019 4,316 5 View

What program can I use for calculating the surfase tension of an aqueous solution, for free? And where?

I tried with opendrop but the program doesn't work, I'm also trying to make a code for MATLAB, but it has some problemas.

03 November 2017 1,671 5 View

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08 August 2017 728 2 View

At high ph (around 11), would cellulase be inhibited or denatured? Would it recovered its activity when I lower the pH again?

I am performing enzymatic saccharification using the enzyme cocktail CTec2. I am hydrolyzing alkaline pretreated lignocellulosic biomass, that I add to the reaction slowly in several steps, since...

08 September 2016 5,877 1 View

How can I remove micro-arc oxidation (MAO) coatings of Ti surfaces to use in XRD measurements?

14 July 2016 7,139 4 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View