Is there any reference for using 70% acetonitrile for washing the columns of HPLC system?
Can we change USP method to In House method for carrying out the assay of any particular dosage form?
10 November 2017 2,743 1 View
If we increase or decrease the injection volume in HPLC will it make any difference in the retention time of the peak?
03 April 2016 2,373 2 View
Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?
03 April 2016 844 0 View
Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?
03 April 2016 3,735 0 View
Is there any material other than caffeine to calibrate the HPLC??
03 April 2016 7,365 4 View
What is the difference between moisture content and water content?
03 April 2016 4,478 4 View
Why do we use methanol as a solvent in KF standardisation? Why not other solvent?
03 April 2016 7,469 0 View
What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
03 April 2016 6,137 1 View
If any mobile phase contains a mixture of organic solvent and buffer, then for how many days can we store it for using it again and again? Also, if the mobile phase contains only the mixture of...
02 March 2016 8,022 8 View
Which is the best solvent used for washing of HPLC columns and in what concentration?
02 March 2016 9,490 6 View
Could you recommend some articles on Urban Transportation System optimization and Innovation?
13 August 2024 2,595 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello everyone What is your opinion about the introduction of an expert decision support system in which the rules are extracted from existing data without human intervention, instead of being...
31 July 2024 5,785 4 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View