I tried isolating bacteriophage for staphylococcus aureus from sewage water, and I did spot test , where they showed a hazy zone of clearance after 3 days again I repeated spot test and got good zone , but after then I tried all days but I never got clearance zone .can anyone tell me what might be the reason , like the storage or the method I follow is not proper .additionally I store the filterate at - 20°.
There can be a lot of reasons for such results, some may include:
1. Contamination of S. aureus culture
2. Low titer lysate
3. Improper stock preparation and storage methods (e.g power break down e.t.c.)
Read this chapter to optimize your protocol. Best of luck!
dear Archana Logan
avoid contamination of S. aureus
store at 4C rather than -20,
S. aureus phages are quite stable about 10 fold decrease in titer occur even stored at 4C for six months
for S.aureus phages isolation and plaque enhancement use TSB medium with addition of CaCl2
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