reason for linearizing the plasmid DNA before transfection?
Do you mean "transfection", perhaps? If not, I don't know where you got that idea from, but I'm pretty sure its wrong!
I agree with Geoff Margison, transformation of linearized plasmid DNA will result in nothing - unless you do a ligation before.
If you indeed mean transfection, it is not strictly necessary to linearize the plasmid.
To add to Sophie's comment, agreed that its not essential, but we used to argue that if we chose the site of linearization to be well outside the expression/selection marker cassette, then the host cell's "choice" would be obviated, and the process would be more likely to work. But of course we never tested that idea experimentally - maybe one of the other 'Gaters has??
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