Dear Colleagues
I am going to do cell differentiation of pre-osteoblast to mature osteoblast by giving induction from my materials.
Is that possible to grow the pre-osteoblasts cell line (MC3T3-E1 Subclone 4 CRL-2593) in the DMEM medium (product code LM 001-05). We have this medium in our lab. However, I saw that a lot of papers used a variety of MEM alpha (recommended by ATCC is MEM alpha without ascorbic acid for base medium, product code A1049001).
If I should use MEM alpha, could you kindly recommend what kind of MEM alpha should I use, please? Do I need to use MEM alpha included ascorbic acid or not? In our lab we have L-ascorbic acid separately (product code 255564).
Thank you and best regards.
Hello! I've worked with MC3T3-E1 cell line for a diverse assays, mainly without differenciating them. The commercial medium you mention is the one I used for culture the cells and perform my tests. I strongly recommend you that medium if you want to grow the cells without any differentiation; it is very effective, the cells grow very fast, showing good morphology and good behavior. If your goal is to differenciate them, I indeed recommend using a medium with ascorbic acid.