Why has no one been able to isolate any part of the SARS-CoV-2 virus except the spike protein from a human source?

01 April 2023 1 4K Report

Other viruses are routinely isolated, but not SARS-CoV-2.

Could it be that SARS-CoV-2 is actually a monkey virus, not infective to humans? Check out this study. Article Culture of SARS-CoV-2 in a panel of laboratory cell lines, p...

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Science method

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Technically: Do proctoring software add to quality assurance in assessment in blended learning modules?

One of the challenges in ensuring quality and integrity in blended learning modules is cheating especially when it comes to assessment (Tests, exams, and assignments). Now one of the solutions...

21 July 2024 6,741 0 View

Did Einstein misunderstand the photo-electric effect?

Photo-electric effect A light particle moving towards a surface cannot (by collision) force an electron particle to move away from that surface. A light wave can instead make interference with a...

19 July 2024 8,389 9 View

Can we find the Gromacs version from XTC file or TRR?

Hello everyone, This soudns like a strange question, but I created five XTC files using five different versions of GROMACS some years ago. All other files are missing, and I want to repeat the...

16 July 2024 7,205 2 View

Two PhD and One Master to expand R&D team, anyone interested?

Hi all, if you are looking for PhD and Master opportunity to work on Microalgae research and Biosensor for bacteria detection. Please do not hesitate to email me [email protected]

07 July 2024 7,576 0 View

Is light quantisation an illusion?

The photo-electric effect does not support quanta in light, since a light particle moving towards a surface cannot cause an electron to move away from the same surface at a right angle. Instead, a...

06 July 2024 2,288 35 View

Is gravity an EMERGENT phenomenon?

Newton found the law of gravity - but did not understand it. Fatio understood the law - but he was not accepted. Fatio assumed fast particles moving in all directions. Stability in planetary...

30 June 2024 9,284 28 View

Any automatic way to outline an image to measure the area in pixels?

Hi, I am trying to measure the area of the caudal fin in zebrafish, to study regeneration. I need to measure the area of the regenerating fin every 3 days after injury. So far, i have been using...

27 June 2024 5,068 1 View

Unstructured Interview Design?

In my qualitative study, I am trying to avoid the traditional belief that types of qualitative research design are just grounded theory, ethnography, phenomenology, case study, historical, and...

15 June 2024 3,884 1 View

Considering the issues with the ERP of GCTU, management has asked that you advise them on cloud computing models.?

Considering the issues with the ERP of GCTU, management has asked that you advise them on cloud computing models. You are expected to give 3 advantages and disadvantages of each of the models....

09 June 2024 5,629 0 View

Can I or you add publications to the page of Adri Jacobs?

Dear ResearchGate, I came across the ResearchGate page of Adri Jacobs, who passed away in 2010. This page contains 25 publication of Adri mainly from the period 2000-2008. One paper (2011) must...

01 June 2024 8,763 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

Why not we are following sars COVID 19 guidance?

If the case of Irak is of December 2020 then i think I can suggest pl follow all the infection cause and death, already in our researchgate.

19 July 2024 3,356 2 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 781 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Does anyone know how to improve calcium-imaging fluorescence with GRIN lenses?

Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...

08 July 2024 5,178 2 View

Why do I have two different bands from the same virus after PCR?

I get two different bands from BCMV after PCR

07 July 2024 2,112 1 View

How to load Remedesivir drug into mesoporous nanoparticles?

Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...

01 July 2024 889 3 View

Problems to find a region of a Virus gene?

We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...

01 July 2024 7,644 3 View