I repeated the experiment and I got the same thing

Dear Omar,

FCCP should depolarize a mitochondrial membrane using just 5uM!

I think you have problem with FCCP loading or the FCCP is old

get new ones

Cheers,

Usama

Dear Usama,

Thanks for your reply. It worked for other experiments and it did not work only with the JC-1, that's mean the stock I have is actually fine.

Dear Omar,

do you got the answer for this question? I've meet the same situation and tried to find the explanation.

Thanks.