After selecting suitable colony, I have extracted through Kit. Then run on gel conforming expression.
May be dimmers
They may be are two form of same plasmids. This link. may help you to understand.
http://www.bioinformatics.nl/molbi/SimpleCloningLab/electrophoresis.htm
It's ok if you are having two bands in your gel. Sometimes it also shows three bands.. Obviously it is contaminated due to some nick in the plasmid during your isolation through Kit. Overall i think it happens
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