Why double bands appear in plasmid (pET 30) gel expression? Is this same plasmid or contamination?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Umer Farooq

May be dimmers

Mrityunjay Kumar Singh

They may be are two form of same plasmids. This link. may help you to understand.

http://www.bioinformatics.nl/molbi/SimpleCloningLab/electrophoresis.htm

Parth Sarathi Nayak

It's ok if you are having two bands in your gel. Sometimes it also shows three bands.. Obviously it is contaminated due to some nick in the plasmid during your isolation through Kit. Overall i think it happens