Separation of rare earth elements by using c18 RP-HPLC, I use NTA,HIBA as eluants, OS as modifier and for detection I use Arsenazo III at 645nm.
There may be some chocking in the column. Use hot water followed by methanol or ACN. This will help you. Try it.
Ok ,already i washing with pure water every day after i finished but i will try your method.Thank you
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