Pyocyanin promotes aggregates/ biofilm formation by P. aeruginosa by recruitment of extracellular DNA (eDNA) and intensification of cell-cell interactions.
Article Pyocyanin Facilitates Extracellular DNA Binding to Pseudomon...
Pyocyanin also has cytotoxic properties against host cells (generation of reactive oxygen species and ROS-dependent initiation of inflammation).
Article Cellular Effects of Pyocyanin, a Secreted Virulence Factor o...
Hello Sarshad, Pawel has done the job well. To add to that, I think the physiological role of pyocyanin could be strain-dependent. See; www.pnas.org/content/pnas/109/41/E2823.full.pdf
The phenazine also alters homeostasis and carbon flux through central metabolic pathways like Entner-Doudoroff pathway and TCA cycle in strain PA14. It also plays a role in signaling for anti-oxidation involving glutathione reduction; It is a terminal signaling factor in the quorum sensing network of Pseudomonas aeruginosa. Your other segment about secondary metabolism is also true. They are mostly produced at the intersection of primary and secondary metabolism hence the roles presented earlier. See https://www.physiology.org/doi/pdf/10.1152/ajplung.00025.2004 http://www.jbc.org/content/early/2017/02/07/jbc.M116.772848.full.pdf. I hope these were useful.