Why does not have flow cytometry (partec) repeatability?

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What is the best way of studying plasmodesma changes in plant cells?

In order to track the efficeincy of treatments on virus elimination in plants, I need to check the plasmodesma changes in plant tissues. Is SEM suitable to follow the changes or the more simple...

02 March 2019 4,039 3 View

Dear Dr. Alkowni, do you recommand DAS-ELISA in olive's viruses detection?

As you are more experienced in virus detection, I have tested DAS-ELISA in olives and I am setting up RT-PCR to evaluate ELISA results. Since I do not have positive controls of all viruses, Would...

01 January 1970 4,271 5 View

Do you have positive controls of olive's viruses?

Dear Dr. Faggioli, I am also working on olive's viruses in Agricultural Biotechnology Research Institute of Iran (ABRII) as my dissertation. In Iran only 4 viruses have been reported in olive's...

01 January 1970 8,059 7 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

Ahmad Ajani

Is it ok to describe more.

Leila Mirzaei

yes of course. I have set up parsley as an standard for my samples (Olive), when I mix the two samples together in order to find to seperate peak, sometimes it does not show two peaks though I am sure that the two peaks do not overlap with each other. I use Yokoya et al (1999).