According to DSMZ and my own flow cytometry results, CD7 expression of NK-92 cell line is 99%. As we know, NK-92 MI cell line is an IL-2 independent NK cell line derived from NK-92 cell line by transfection. The parental cells were transfected with human IL-2 cDNA in the retroviral MFG-hIL-2 vector by particle-mediated gene transfer. I bought NK-92 MI from ATCC and found MI negative for CD7 expression. I searched the primary article Characterization of Genetically Altered, Interleukin 2-Independent Natural Killer Cell Lines Suitable for Adoptive Cellular Immunotherapy(pdf attached) and found the comparison part between NK-92 and NK-92 MI didn't mention CD7, which is so weird because the author examined a series of surface markers including CD1, CD2, CD3, CD4, CD5, CD8, CD10, CD11a (LFA-1), CD14, CD16, CD19, CD20, CD23, CD25 (IL-2 receptor), CD28, CD34, CD45, CD50 (ICAM-3), CD54 (ICAM-1), CD56, CD105 (ICAM-2), CD122 (IL-2 receptor, b chain), and HLA-DR.
I am wondering the difference between CD7 expression in NK-92 and MI cell line since I found it rather hard for MI to be transducted with pcDH-based CD7 lentivirus. (MI can be successfuly transducted with pcDH-vector, but after countless trails no GFP can be detected in MI transducted with CD7 lentivirus) I used the same plasmid and packaged same virus to transduct NK-92 and T cells, which both highly express CD7, and it did work. Since theoretically the only difference between NK-92 and MI is IL-2 independence and CD7 expression, I want to know whether there is a relationship among IL-2, CD7 and tranduction efficiency.
Thanks so much if anyone can offer advice or ideas.
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