I used 'Porcinetrace ELISA Kit - Gelatin' (basically the kit is used to detect the presence of porcine DNA) and I tested this on bovine skin gelatin with high gel bloom (strength) - it shows false positive results. Tried it few more times and the results sometimes show false positive and negative results. However, the analysis displays negative results on bovine skin gelatin with low gel bloom. Anyone have any idea why high gel bloom of bovine skin gelatin give false positive results - despite it not containing any trace of porcine DNA?
Hi Nyca, this ELISA kit purportedly detects porcine gelatin and not porcine DNA.
https://pop-bio.com/product/porcinetrace-elisa-kit-gelatin/?v=7516fd43adaa
Steingrimur Stefansson ah! Yes! Porcine gelatin! I’m confusing myself again, but thank you for correcting me... any idea why bovine skin with high gel bloom give false positive results?
Collagen (I) is a well conseved protein across species. Since gelatin is made by hydrolyzing collagen, my guess is that high bloom gelatin is less hydrolyzed and shows some conserved epitopes.
Steingrimur Stefansson My senior did test fish skin gelatin with high gel bloom but it still didn't give false results. So the problem must be the bovine itself? Is there any article regarding this matter?
Well, fish are more distant from bovine that porcine from bovine. Maybe there are epitopes that are conserved in the mammalian collagen.
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