I am using it for intracellular staining. Why does it have to be made fresh every time?
Due to the presence of fetal bovine serum as one of its primary constituent it is suggested to prepare it fresh everytime
I need to do immunohistochemistry in mouse brain, particularly in neurotensin-cre mouse line.
22 May 2024 3,565 7 View
Dear experts, I am looking for someone interested in determining and possibly describing the new species in the photo (probably a Hirsutiella sp., Arachnida, Trombidiformes, Trombiculidae). The...
27 March 2024 9,244 2 View
Research publication details and processes
20 March 2024 3,140 2 View
Dear all, I am trying to functionalize crosslinked PVA by esterification using Mukaiyama reagent (Please see scheme attached). I have tried Dimethylcarbonate as a solvent, but PVA did not swell...
23 January 2024 2,381 1 View
Dear colleagues, could someone tell me the cut-off points of the Buss and Perry Aggression Questionnaire to distinguish between healthy and pathological subjects?
07 January 2024 890 2 View
i am looking for a protocol to isolate cytosol and membranes from cultured primary neurons
04 January 2024 975 0 View
I'm a Venezuelan PE Teacher working in a British International School at Caracas, Venezuela and one of my Appraisal Projects is to Adapt the UK National Curriculum to the Venezuelan Context in...
01 November 2023 7,149 0 View
I have a CT scan of a human skull. I´ve edited, performing a maxillary orthognathic surgery (Le Fort I), and added two plates with 4 screws each. I´ve export this set as a whole and separated with...
10 September 2023 8,370 4 View
Hello, I am planning an experiment to measure enzimatic activity, and i will take sample every 24hrs. The only problem is that substrate is non soluble... nevertheless, enzyme can still access to...
21 June 2023 9,481 4 View
We want to study immune responses in mice treated with a lipophilic compound that has to be dissolved in DMSO. Given the toxicity and potential effect on immune response due to administration of...
14 June 2023 4,862 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View