Why do we use 16 days pregnant mouse?

07 July 2017 1 964 Report

When I experiment to get cerebral cortex from pups of pregnant mouse, protocol says that we should use 16 days pregnant mouse for the experiment. Why?? Please answer in detail😀😀

I get the cortex to culture and get protein controlled by me for western blot

Davide Aprile

Hi!

I think you will find the answer you're looking for in this paper.

Culturing hippocampal neurons - Kaech & Banker Nature protocols (2007)

It is focused on hippo neurons but the reasons described here can fit very well also for cortical cultures.

Cheers

Davide

Badges
Science method

More Kim Seong Bin's questions See All

Phase I study of chemotherapy drug??

Hello, I have read some papers about hematologic malignancies. And, I wondered why investigators compare short-term infusion and continuous infusion during phase I study. And also, I wondered why...

03 April 2018 1,966 3 View

Why should we make agarose gel by hand and no sell?

Why should we make agarose gel by hand and no sell? Is it related to properties of gel?

07 August 2017 6,771 0 View

Cleanig agent for glassware

Are acetone, methanol, and ethanol proper as cleaning agents for glassware? In addition to volatility, solubility, and low cost, what kinds of reason that ensure them as good cleaning agents?

07 August 2017 2,544 3 View

Why do we use non-endotoxin-free plasmid prep kit?

We mainly use endotoxin-free kit for plasmid preparation due to disturbance of LDS. But there are also non-endotoxin-free kit. Why do we use it? I think that non-endotoxin-free kit is cheaper than...

07 August 2017 1,401 3 View

Why endotoxin-free in plasmid DNA prep?

When we prepare plasmid DNA with some tools like maxi prep., endotoxin-free is very improtant. In terms of transfection efficiency, I guess that LDS may compete with DNA as transfection reagents,...

07 August 2017 5,398 5 View

What is explanation about homologous recombination in brief?

I have studied translocation of exogenous DNA during transformation of E.coli But, I cannot understand homologous recombination of ssDNA and relationship among RecA, DprA, and ssDNA Can you...

07 August 2017 456 1 View

Why do we heat fly head for protein extract in western blotting

If we extract protein in heads of flies, we homogenize the heads and put LDS buffer and reducing agents. And then, we put them in heating block Why do we heat the homogenized heads in heating...

06 July 2017 7,050 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

How can I add a new WSN routing protocol in NS2 or NS3?

I'm trying to find in literature a way to help me adding a modified Yen's algorithm in ns2 or its variance ns3 or another simulator. Have you some tips which can be helpful, I'm opening in the...

19 February 2021 7,981 3 View