Why do we use 16 days pregnant mouse?

07 July 2017 1 961 Report

When I experiment to get cerebral cortex from pups of pregnant mouse, protocol says that we should use 16 days pregnant mouse for the experiment. Why?? Please answer in detail😀😀

I get the cortex to culture and get protein controlled by me for western blot

Davide Aprile

Hi!

I think you will find the answer you're looking for in this paper.

Culturing hippocampal neurons - Kaech & Banker Nature protocols (2007)

It is focused on hippo neurons but the reasons described here can fit very well also for cortical cultures.

Cheers

Davide

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