Why should we make agarose gel by hand and no sell?
Is it related to properties of gel?
Hello, I have read some papers about hematologic malignancies. And, I wondered why investigators compare short-term infusion and continuous infusion during phase I study. And also, I wondered why...
03 April 2018 2,009 3 View
Are acetone, methanol, and ethanol proper as cleaning agents for glassware? In addition to volatility, solubility, and low cost, what kinds of reason that ensure them as good cleaning agents?
07 August 2017 2,611 3 View
When we prepare plasmid DNA with some tools like maxi prep., endotoxin-free is very improtant. In terms of transfection efficiency, I guess that LDS may compete with DNA as transfection reagents,...
07 August 2017 5,519 5 View
We mainly use endotoxin-free kit for plasmid preparation due to disturbance of LDS. But there are also non-endotoxin-free kit. Why do we use it? I think that non-endotoxin-free kit is cheaper than...
07 August 2017 1,463 3 View
I have studied translocation of exogenous DNA during transformation of E.coli But, I cannot understand homologous recombination of ssDNA and relationship among RecA, DprA, and ssDNA Can you...
07 August 2017 504 1 View
If we extract protein in heads of flies, we homogenize the heads and put LDS buffer and reducing agents. And then, we put them in heating block Why do we heat the homogenized heads in heating...
06 July 2017 7,110 2 View
When I experiment to get cerebral cortex from pups of pregnant mouse, protocol says that we should use 16 days pregnant mouse for the experiment. Why?? Please answer in detail😀😀 I get the cortex...
06 July 2017 974 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View