Phase I study of chemotherapy drug??

Hello, I have read some papers about hematologic malignancies. And, I wondered why investigators compare short-term infusion and continuous infusion during phase I study. And also, I wondered why...

03 April 2018 1,966 3 View

Cleanig agent for glassware

Are acetone, methanol, and ethanol proper as cleaning agents for glassware? In addition to volatility, solubility, and low cost, what kinds of reason that ensure them as good cleaning agents?

07 August 2017 2,544 3 View

Why do we use non-endotoxin-free plasmid prep kit?

We mainly use endotoxin-free kit for plasmid preparation due to disturbance of LDS. But there are also non-endotoxin-free kit. Why do we use it? I think that non-endotoxin-free kit is cheaper than...

07 August 2017 1,401 3 View

Why endotoxin-free in plasmid DNA prep?

When we prepare plasmid DNA with some tools like maxi prep., endotoxin-free is very improtant. In terms of transfection efficiency, I guess that LDS may compete with DNA as transfection reagents,...

07 August 2017 5,398 5 View

What is explanation about homologous recombination in brief?

I have studied translocation of exogenous DNA during transformation of E.coli But, I cannot understand homologous recombination of ssDNA and relationship among RecA, DprA, and ssDNA Can you...

07 August 2017 456 1 View

Why do we heat fly head for protein extract in western blotting

If we extract protein in heads of flies, we homogenize the heads and put LDS buffer and reducing agents. And then, we put them in heating block Why do we heat the homogenized heads in heating...

06 July 2017 7,050 2 View

Why do we use 16 days pregnant mouse?

06 July 2017 933 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View