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Questions related from Kim Seong Bin
Hello, I have read some papers about hematologic malignancies. And, I wondered why investigators compare short-term infusion and continuous infusion during phase I study. And also, I wondered why...
04 April 2018 1,997 3 View
I have studied translocation of exogenous DNA during transformation of E.coli But, I cannot understand homologous recombination of ssDNA and relationship among RecA, DprA, and ssDNA Can you...
08 August 2017 489 1 View
When we prepare plasmid DNA with some tools like maxi prep., endotoxin-free is very improtant. In terms of transfection efficiency, I guess that LDS may compete with DNA as transfection reagents,...
08 August 2017 5,502 5 View
We mainly use endotoxin-free kit for plasmid preparation due to disturbance of LDS. But there are also non-endotoxin-free kit. Why do we use it? I think that non-endotoxin-free kit is cheaper than...
08 August 2017 1,450 3 View
Why should we make agarose gel by hand and no sell? Is it related to properties of gel?
08 August 2017 6,798 0 View
Are acetone, methanol, and ethanol proper as cleaning agents for glassware? In addition to volatility, solubility, and low cost, what kinds of reason that ensure them as good cleaning agents?
08 August 2017 2,598 3 View
When I experiment to get cerebral cortex from pups of pregnant mouse, protocol says that we should use 16 days pregnant mouse for the experiment. Why?? Please answer in detail😀😀 I get the cortex...
07 July 2017 964 1 View
If we extract protein in heads of flies, we homogenize the heads and put LDS buffer and reducing agents. And then, we put them in heating block Why do we heat the homogenized heads in heating...
07 July 2017 7,098 2 View
