Can focused ion beams be used for cutting 500nm wide trenches in metals, and ceramics?

Does anyone have relevant info on this?

01 May 2019 1,045 3 View

What is the geometry of photometry bench baffle?

In a typical photometry bench (light source at one, photometer under test at the other), is the geometry of the baffle critical? If so, how does one design such baffles? EDIT. Thank you all for...

27 April 2018 4,774 3 View

What is the best effect size estimator for ecological data with low sample size?

13 May 2017 8,162 4 View

How to quantify DNA before and after amplification?

I run a PCR with the sputum product which was microscopy positive, but after PCR the result was negative.

03 March 2017 9,847 3 View

Hi, is it possible to make or pour gradient agarose electrophoresis gels. Has someone got an optimised protocol or article that I can follow ?

Used for the separation of large aggregates.

22 February 2017 4,674 3 View

ROS study in GFP positive cells?

10 January 2017 5,465 3 View

Is there available a philological commentary of the Greek text of the Letter to Menoeceus ?

23 June 2016 7,863 4 View

Two [sets of] questions: one on conceptual structure, the other on conceptual development. Both regarding the primitive/complex concept distinction.

On conceptual structure: Are some concepts built out of (and, therefore, reducible to) other concepts? Is this the case only within particular theories of concepts (e.g. the Classical Theory,...

02 June 2016 1,792 3 View

No title

13 August 2013 3,887 3 View

How do we define influencers in influencer marketing?

Hi all, so much has been said and written about the role of influencers in influencer marketing. So far, most studies focus on influencers in terms of their number of followers, thus we have nano,...

01 January 1970 6,419 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View