Hi, I am currently doing single-unit recordings on mice visual cortex. I noticed that in some cases, the firing properties of neurons changed greatly. When I initially find the cell, it is quite sensitive to light stimulation, has obvious orientation selectivity, and has low spontaneous firing rate. However, after 1-2 hours of recording, the cell's spontaneous firing rate increases a lot, and lose the orientation selectivity partly or even entirely. The mouse is anaesthetized by isoflurane and the heart rate is kept between 360-420 beats/min. I wish to know if there is anything else that I need to take care of, and if this phenomenon suggests anything wrong with the cortex. Thanks in advance.
What kind of electrodes are you using?
Do you get any change in the spike waveform over this time?
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