Hello,
I have performed WB analysis and qPCR analysis to analyze the protein level and the rispective gene relative expression of mTOR, GBA1, LC3 genes in brain of mutant mice.
I observed a significant downregulation of all these genes in the mutant mouse compared to WT mice, but no alterations in the protein quantity. Moreover, I did the same for LAMP2 protein and transcripts levels. LAMP2 protein levels were increased, which its mRNA levels were downregulated.
Have anyone experienced this type of "compensation" mechanism between trascriptional regulation and protein levels?
Which kind of regulation can be responsible for this differences?
Thank you so much
This is weird. Have you checked that the wb signals fall within the dynamic range of the assay? Also, when you say "significantly downregulated", how significantly is that?
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